Publication: Sexually dimorphic crosstalk at the maternal-fetal interface

Tianyanxin Sun*, Tania L Gonzalez*, Nan Deng, Rosemarie DiPentino, Ekaterina L Clark, Bora Lee, Jie Tang, Yizhou Wang, Barry R Stripp, Changfu Yao, Hsian-Rong Tseng, S Ananth Karumanchi, Alexander F Koeppel, Stephen D Turner, Charles R Farber, Stephen S Rich, Erica T Wang, John Williams, III, Margareta D Pisarska. 

"Sexually dimorphic crosstalk at the maternal-fetal interface". The Journal of Clinical Endocrinology & Metabolism, 105(12): e4831-e4847, 2020, https://doi.org/10.1210/clinem/dgaa503

*Co-first authors.

Links

• PubMed ID: 32772088
• Journal of Clinical Endocrinology & Metabolism research article
• Receptor-ligand gene expression
• Cell markers of placenta cell types
• Cell markers of trophoblast cell types within placenta (subanalysis)
• Gene expression differences due to fetal sex
• Preprint at bioRxiv
• Includes additional information about the bulk RNA-seq of first trimester maternal-fetal interface using matched maternal decidua and placenta (CVS) RNA-seq
• Includes additional details about the single cell RNA-seq tSNE plots and cell developmental trajectories
• Sequencing data deposited into NCBI GEO superseries GSE131875
• GSE131696: Single cell RNA-sequencing of CVS samples
• N=6 placenta at late first trimester, 3 female and 3 male
• See preprint for extended details on the cell differentiation trajectory of cytotrophoblasts (stem cell-like) into extravillous trophoblasts (migratory/invasive cells) and syncytiotrophoblasts (multi-nuclei fused cells that act as a protective barrier in placenta).
• GSE131874: Total RNA-sequencing of matched maternal decidua and CVS tissue
• N=4/group, 2 pregnancies with female fetus, 2 pregnancies with male fetus
• N=8 samples total, 4 placenta and 4 decidua from 4 pregnancies
• See preprint for matched CVS vs decidua differential gene expression analysis
• We did not focus on this as much in the published version
• All pregnancies continued to full term delivery & live births
• Supplemental Figures deposited into FigShare.com
• Collection: https://doi.org/10.6084/m9.figshare.c.4741742.v2
• Figure S1 = PCA plot of matched chorionic villi and maternal decidua (bulk RNA-seq)
• Figure S2 = Heatmaps of differentially expressed genes from pairwide comparison of placental cell clusters (single cell analysis)
• Table S1 = Patient demographics and pregnancy outcomes
• 4 pregnancies for bulk RNA-seq
• 6 pregnancies for single cell RNA-seq
• 7 pregnancies for immunohistochemistry to validate single cell RNA-seq
• Table S2 = Placental cell markers from single cell RNA sequencing
• Table S3 = Placental cell type-specific sexually dimorphic genes
• Table S4 = Placenta receptors and ligands sexually dimorphic in both bulk and single cell RNA sequencing
• Table S5 = Trophoblast subcluster markers in human first trimester
• Links to figshare.com are correct in the final published version, but not the pre-proof PubMed Central version.